Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr class="thead"><td class="align_left">Antigen</td><td class="align_center">Species</td><td class="align_center">Clone</td><td class="align_center">Dilution</td><td class="align_center">Provider</td><td class="align_center">Protocol specifications</td></tr><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr><td class="align_left">Akt</td><td class="align_center">Rabbit</td><td class="align_center">Polyclonal</td><td class="align_center">1 : 1000</td><td class="align_center">Abcam (ab8805)</td><td class="align_center">—</td></tr><tr><td class="align_left">CD4</td><td class="align_center">Rabbit</td><td class="align_center">Monoclonal</td><td class="align_center">1 : 50</td><td class="align_center">Abcam (ab133616)</td><td class="align_center">EDTA <svg height="11.5173pt" id="M9" style="vertical-align:-3.129319pt" version="1.1" viewbox="-0.0498162 -8.38798 38.1192 11.5173" width="38.1192pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M169 380V459C122 440 66 423 24 416V392C86 384 90 382 90 317V-135C90 -201 81 -207 17 -213V-240H253V-213C176 -207 169 -201 169 -125V6C182 -1 208 -11 238 -12C368 12 487 109 487 260C487 358 421 449 310 449C298 449 279 444 261 433L169 380ZM169 346C196 367 237 389 269 389C341 389 403 329 403 221C403 109 347 37 263 37C228 37 191 53 169 76V346Z" id="g121-110"></path></g><g transform="matrix(.013,0,0,-0.013,6.812,0)"><path d="M729 650H468V622C546 615 554 610 554 527V365H213V527C213 610 221 616 297 622V650H38V622C120 616 128 611 128 527V123C128 39 120 34 40 28V0H303V28C221 34 213 40 213 123V322H554V123C554 39 546 34 460 28V0H728V28C647 34 639 39 639 123V527C639 610 647 615 729 622V650Z" id="g121-70"></path></g><g transform="matrix(.013,0,0,-0.013,20.402,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path></g><g transform="matrix(.013,0,0,-0.013,31.665,0)"><path d="M244 635C114 635 38 519 38 422C38 317 111 240 217 240C236 240 255 244 277 256L345 292C311 140 203 39 59 15L64 -15C89 -15 150 -5 204 17C339 72 440 202 440 386C440 521 368 635 244 635ZM228 602C326 602 352 479 352 390C352 370 351 347 348 324C327 308 293 296 258 296C174 296 124 369 124 458C124 517 152 602 228 602Z" id="g113-58"></path></g></svg> before incubation with blocking solution</td></tr><tr><td class="align_left">CD8</td><td class="align_center">Rabbit</td><td class="align_center">Polyclonal</td><td class="align_center">1 : 25</td><td class="align_center">Abcam (ab4055)</td><td class="align_center">EDTA <svg height="11.5173pt" id="M10" style="vertical-align:-3.129319pt" version="1.1" viewbox="-0.0498162 -8.38798 38.1192 11.5173" width="38.1192pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M169 380V459C122 440 66 423 24 416V392C86 384 90 382 90 317V-135C90 -201 81 -207 17 -213V-240H253V-213C176 -207 169 -201 169 -125V6C182 -1 208 -11 238 -12C368 12 487 109 487 260C487 358 421 449 310 449C298 449 279 444 261 433L169 380ZM169 346C196 367 237 389 269 389C341 389 403 329 403 221C403 109 347 37 263 37C228 37 191 53 169 76V346Z" id="g121-110"></path></g><g transform="matrix(.013,0,0,-0.013,6.812,0)"><path d="M729 650H468V622C546 615 554 610 554 527V365H213V527C213 610 221 616 297 622V650H38V622C120 616 128 611 128 527V123C128 39 120 34 40 28V0H303V28C221 34 213 40 213 123V322H554V123C554 39 546 34 460 28V0H728V28C647 34 639 39 639 123V527C639 610 647 615 729 622V650Z" id="g121-70"></path></g><g transform="matrix(.013,0,0,-0.013,20.402,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path></g><g transform="matrix(.013,0,0,-0.013,31.665,0)"><path d="M244 635C114 635 38 519 38 422C38 317 111 240 217 240C236 240 255 244 277 256L345 292C311 140 203 39 59 15L64 -15C89 -15 150 -5 204 17C339 72 440 202 440 386C440 521 368 635 244 635ZM228 602C326 602 352 479 352 390C352 370 351 347 348 324C327 308 293 296 258 296C174 296 124 369 124 458C124 517 152 602 228 602Z" id="g113-58"></path></g></svg> before incubation with blocking solution</td></tr><tr><td class="align_left">CD19</td><td class="align_center">Mouse</td><td class="align_center">Monoclonal</td><td class="align_center">1 : 200</td><td class="align_center">Abcam (ab31947)</td><td class="align_center">—</td></tr><tr><td class="align_left">HIF-1<i>α</i></td><td class="align_center">Mouse</td><td class="align_center">Monoclonal</td><td class="align_center">1 : 800</td><td class="align_center">Abcam (ab16066)</td><td class="align_center">EDTA <svg height="11.5173pt" id="M11" style="vertical-align:-3.129319pt" version="1.1" viewbox="-0.0498162 -8.38798 38.1192 11.5173" width="38.1192pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M169 380V459C122 440 66 423 24 416V392C86 384 90 382 90 317V-135C90 -201 81 -207 17 -213V-240H253V-213C176 -207 169 -201 169 -125V6C182 -1 208 -11 238 -12C368 12 487 109 487 260C487 358 421 449 310 449C298 449 279 444 261 433L169 380ZM169 346C196 367 237 389 269 389C341 389 403 329 403 221C403 109 347 37 263 37C228 37 191 53 169 76V346Z" id="g121-110"></path></g><g transform="matrix(.013,0,0,-0.013,6.812,0)"><path d="M729 650H468V622C546 615 554 610 554 527V365H213V527C213 610 221 616 297 622V650H38V622C120 616 128 611 128 527V123C128 39 120 34 40 28V0H303V28C221 34 213 40 213 123V322H554V123C554 39 546 34 460 28V0H728V28C647 34 639 39 639 123V527C639 610 647 615 729 622V650Z" id="g121-70"></path></g><g transform="matrix(.013,0,0,-0.013,20.402,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path></g><g transform="matrix(.013,0,0,-0.013,31.665,0)"><path d="M244 635C114 635 38 519 38 422C38 317 111 240 217 240C236 240 255 244 277 256L345 292C311 140 203 39 59 15L64 -15C89 -15 150 -5 204 17C339 72 440 202 440 386C440 521 368 635 244 635ZM228 602C326 602 352 479 352 390C352 370 351 347 348 324C327 308 293 296 258 296C174 296 124 369 124 458C124 517 152 602 228 602Z" id="g113-58"></path></g></svg> before incubation with blocking solution</td></tr><tr><td class="align_left">HIF-2<i>α</i></td><td class="align_center">Mouse</td><td class="align_center">Monoclonal</td><td class="align_center">1 : 2000</td><td class="align_center">Abcam (ab8365)</td><td class="align_center">EDTA <svg height="11.5173pt" id="M12" style="vertical-align:-3.129319pt" version="1.1" viewbox="-0.0498162 -8.38798 38.1192 11.5173" width="38.1192pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M169 380V459C122 440 66 423 24 416V392C86 384 90 382 90 317V-135C90 -201 81 -207 17 -213V-240H253V-213C176 -207 169 -201 169 -125V6C182 -1 208 -11 238 -12C368 12 487 109 487 260C487 358 421 449 310 449C298 449 279 444 261 433L169 380ZM169 346C196 367 237 389 269 389C341 389 403 329 403 221C403 109 347 37 263 37C228 37 191 53 169 76V346Z" id="g121-110"></path></g><g transform="matrix(.013,0,0,-0.013,6.812,0)"><path d="M729 650H468V622C546 615 554 610 554 527V365H213V527C213 610 221 616 297 622V650H38V622C120 616 128 611 128 527V123C128 39 120 34 40 28V0H303V28C221 34 213 40 213 123V322H554V123C554 39 546 34 460 28V0H728V28C647 34 639 39 639 123V527C639 610 647 615 729 622V650Z" id="g121-70"></path></g><g transform="matrix(.013,0,0,-0.013,20.402,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path></g><g transform="matrix(.013,0,0,-0.013,31.665,0)"><path d="M244 635C114 635 38 519 38 422C38 317 111 240 217 240C236 240 255 244 277 256L345 292C311 140 203 39 59 15L64 -15C89 -15 150 -5 204 17C339 72 440 202 440 386C440 521 368 635 244 635ZM228 602C326 602 352 479 352 390C352 370 351 347 348 324C327 308 293 296 258 296C174 296 124 369 124 458C124 517 152 602 228 602Z" id="g113-58"></path></g></svg> before incubation with blocking solution</td></tr><tr><td class="align_left">PI3K</td><td class="align_center">Mouse</td><td class="align_center">Monoclonal</td><td class="align_center">1 : 500</td><td class="align_center">Abcam (ab86714)</td><td class="align_center">—</td></tr><tr><td class="align_left">mTOR</td><td class="align_center">Rabbit</td><td class="align_center">Polyclonal</td><td class="align_center">1 : 500</td><td class="align_center">Abcam (ab1093)</td><td class="align_center">—</td></tr><tr class="table-tr"><td colspan="6"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Primary antibodies that were used and their dilutions.</div>

Oxidative Medicine and Cellular Longevity

tab1

Table 1

Table 1: Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging 

Table 1 | Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging 