Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging

Table 3

Primers used for RT-qPCR: sequences and binding temperatures (Temp).

Gene	Sequence fwd (5 → 3)	Sequence rev (5 → 3)	Temp (°C)
GAPDH	GGA AGG TGA AGG TCG GAG TCA	GTC ATT GAT GGC AAC AAT ATC CAC T	60
Akt	TGT CTC GTG AGC GCG TGT TTT	CCG TTA TCT TGA TGT GCC CGT C	60
HIF-1α	ACG TGT TAT CTG TCG CTT TGA G	ATC GTC TGG CTG CTG TAA TAA TG	59
HIF-2α	ACC CAG TAC CAG GAC TAC AGC	GGC ACG TTC ACC TCA CAG TC	61
PI3K	CTT GCC TCC ATT CAC CAC CTC T	GCC TCT AAT CTT CTC CCT CTC CTT C	60
mTOR	ATC CAG ACC CTG ACC CAA AC	TCC ACC CAC TTC CTC ATC TC	60