Ethyl Acetate Fraction of <i>Hemerocallis citrina</i> Baroni Decreases Tert-butyl Hydroperoxide-Induced Oxidative Stress Damage in BRL-3A Cells

<div><i>Hemerocallis citrina</i> Baroni ethyl acetate fraction (HCEA) prevents t-BHP-induced apoptosis. (a) DAPI staining observed under a fluorescence microscope at 40× magnification. (b) Quantification of BRL-3A apoptotic cells using a flow cytometer with Annexin V-FITC/PI staining. (c) qRT-PCR analysis of expression of apoptosis-related genes. Different lowercase letters indicate significant differences (<span class="nowrap"><svg height="9.272905pt" id="M21" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.049816 -8.635905 44.839329 9.272905" width="44.839329pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.683,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"></path></g><g transform="matrix(.013,0,0,-0.013,22.974,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,29.238,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,32.213,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,38.476,0)"><path d="M153 550H386L412 615L406 623H120L82 318C104 327 142 338 184 338C294 338 347 275 347 187C347 112 305 39 221 39C160 39 119 71 97 89C88 97 80 96 71 90C59 80 50 67 49 57C48 45 52 36 66 23C80 9 123 -12 169 -12C221 -11 288 15 342 59C403 109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z"></path></g></svg>).</span> DAPI: 4<span class="nowrap"><svg height="11.485935pt" id="M22" style="vertical-align:-0.04981709pt" version="1.1" viewbox="-0.049816 -11.436118 3.895168 11.485935" width="3.895168pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>,</span>6-diamidino-2-phenylindole; qRT-PCR: quantitative real-time reverse transcription-polymerase chain reaction; FITC: fluorescein isothiocyanate; PI: propidium iodide.</div>

Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Ethyl Acetate Fraction of <i>Hemerocallis citrina</i> Baroni Decreases Tert-butyl Hydroperoxide-Induced Oxidative Stress Damage in BRL-3A Cells 

Oxidative Medicine and Cellular Longevity

Ethyl Acetate Fraction of Hemerocallis citrina Baroni Decreases Tert-butyl Hydroperoxide-Induced Oxidative Stress Damage in BRL-3A Cells

Figure 5