Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 1

Research Article

Mesenchymal Stem Cells from Cervix and Age: New Insights into CIN Regression Rate

Figure 1

Morphology and expression of genes related to stemness. (a) Phase-contrast images of MSCs derived from the cervixes of younger (yC-MSCs, left) and older patients (oC-MSCs, right) after 14 days of culture. Scale bar, 100 μm. The expression of selected markers related to self-renewal and differentiation potential (OCT4, SOX2, NANOG, and KLF4) was evaluated by RT-PCR (b) and cytofluorimetry (c). (b) Levels of expression detected in yC-MSCs are referred to as -fold with respect to oC-MSCs (expressed as equal to 1). Data are means ± SD from analyses performed on three separate experiments in triplicate. for yC-MSCs versus oC-MSCs. (c) Representative FACScan analyses of cell-surface antigen expression, as indicated. Black histograms refer to oC-MSCs and red histograms to yC-MSCs.
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