Mesenchymal Stem Cells from Cervix and Age: New Insights into CIN Regression Rate

Figure 5

Proliferation and expression of genes related to tumorigenesis in HeLa cells after indirect coculture with MSCs. HeLa cells were indirectly cocultured for 72 hours with yC-MSCs (HeLa/yC-MSCs) or oC-MSCs (HeLa/oC-MSCs). HeLa cultured alone were used as control. (a) Proliferation was assessed by an automated cell counter; for HeLa/yC-MSCs or HeLa/oC-MSCs versus HeLa and for HeLa/yC-MSCs versus HeLa/oC-MSCs. (b) The histogram displays the expression of selected genes related to specific cellular mechanisms, such as oncogenesis (cFOS, cJUN, and cMET), invasion and migration (MMP11), proliferation (mKI67), and angiogenesis (VEGF, CXCL12) in HeLa cells after cocultures with the yC-MSCs and oC-MSCs. Levels of expression detected in cocultured HeLa are referred to as -fold with respect to individually cultured HeLa (expressed as 1). for HeLa/yC-MSCs or HeLa/oC-MSCs versus HeLa and for HeLa/yC-MSCs versus HeLa/oC-MSCs.

(a)

(b)