Protection of Kaempferol on Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage

Figure 2

Antiapoptotic effects of kaempferol on ARPE-19 cells against oxidative stress induced by H₂O₂. (a) ARPE-19 cells with or without H₂O₂ treatment for 24 h, then cells were treated with or without kaempferol (20 or 50 nM/mL) for another 24 h, the cells were then stained with annexin V/PI double staining and analyzed by flow cytometry. The percentage of cells in each quadrant is presented. (b) The quantification of annexin V-positive cells (Q2 + Q4) was calculated for each group cells and are shown in the bar graph. (c) The quantification of early apoptotic cells (Q4) was calculated for each group cells and are shown in the bar graph. The data in (b) and (c) are shown as mean ± SD, , , vs. the samples treated with H₂O₂ alone (marked by black frame).

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