Protection of Kaempferol on Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage

Figure 4

Effects of kaempferol on the protein expression levels of Bcl-2, Bax, and caspase 3 in H₂O₂-treated ARPE-19 cells. ARPE-19 cells were pretreated with or without 1 mM H₂O₂ for 24 h, followed by a 24 h exposure to kaempferol (20 or 50 nM). (a) The cell lysates of control (non-H₂O₂ treatment), H₂O₂-treated, and kaempferol-treated samples were analyzed by Western blot assay with the antibodies of Bcl-2, Bax, caspase 3, and β-actin, respectively. Quantification data of these protein expression levels were measured and normalized to β-actin expression levels. The fold changes vs. control sample were presented in bar graph from (b)–(d), respectively. Data are shown as mean ± SD, , , vs. the sample marked with black frame (H₂O₂ treatment alone), .

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