Protection of Kaempferol on Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage

Figure 5

Kaempferol significantly decreased the ROS activity while increased SOD activity in H₂O₂-treated ARPE-19 cells. (a) ARPE-19 cells were pretreated with or without 1 mM H₂O₂ for 24 h, followed by a 24 h exposure to kaempferol. The intracellular reactive oxygen species (ROS) activities were measured by fluorescence microscope. (b) ARPE-19 cells were pretreated with or without 1 mM H₂O₂ for 24 h, followed by a 24 h exposure to kaempferol; the activities of cellular SOD were measured by a SOD activity assay kit. The SOD levels were normalized against per milligram protein. Data were shown as mean ± SD (). , , vs. H₂O₂ only treated group (marked by black frame).

(a)

(b)