Protection of Kaempferol on Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage

Figure 6

Pretreatment of kaempferol significantly improved sodium iodate-induced retinal damage in SD rats. The rats were anesthetized by intraperitoneal injection of chloral hydrate, sodium iodate (40 mg/kg) was given via the sublingual vein, and the kaempferol was injected into vitreous cavity 3 days before sodium iodate injection. After 7 days of sodium iodate injection, each group rats were analyzed by (a) retinal HE staining; (b) histological analysis of RPE cell layer using confocal microscope through stretched preparation of RPE-choroid-sclera complex tissue; (c) retinal immunofluorescence staining with RPE65 antibody. Experimental groups were: (A) normal control; (B) solvent control; (C) NaIO₃ injection model; (D) kaempferol pretreatment before NaIO₃ injection. Scale bar is 500 μ m.

(a)

(b)

(c)