Protection of Kaempferol on Oxidative Stress-Induced Retinal Pigment Epithelial Cell Damage

Figure 8

Effect of kaempferol on the expressions of VEGF and PEDF. (a) ARPE-19 cells were pretreated with or without 1 mM H₂O₂ for 24 h, followed by a 24 h exposure to kaempferol (20 or 50 nM). The mRNA expression levels of VEGF and PEDF were analyzed by real-time PCR, and the fold changes of each gene were shown in the bar graph compared with the control samples (nontreated cells, value as 1). The data are shown as mean ± SD, , vs. the sample marked with black frame (H₂O₂ treatment alone). (b) Effect of kaempferol on the expressions of VEGF in retinal tissue. Retinal immunofluorescence staining with VEGF antibody was performed in each experimental groups: (A) normal control; (B) solvent control; (C) NaIO₃ injection model; (D) kaempferol pretreatment before NaIO₃ injection. Scale bar is 500 μm.

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