Modulation of Adipocyte Differentiation and Proadipogenic Gene Expression by Sulforaphane, Genistein, and Docosahexaenoic Acid as a First Step to Counteract Obesity

Table 1

Primer sequences used in qPCR.

Gene	Forward primer	Reverse primer	Probe number
Target genes
PPARγ1	GAAAGACAACGGACAAATCACC	GGGGGTGATATGTTTGAACTTG	7
PPARγ2	TGCTGTTATGGGTGAAACTCTG	CTGTGTCAACCATGGTAATTTCTT	2
C/EBPα	AAACAACGCAACGTGGAGA	GCGGTCATTGTCACTGGTC	67
GLUT4	GACGGACACTCCATCTGTTG	GCCACGATGGAGACATAGC	5
Reference genes
ACTB	GTGGGAGAGCAAGGAAGAGA	CACTCTTGGCCCAGTCTACG	56
HPRT1	TCCTCCTCAGACCGCTTTT	CCTGGTTCATCATCGCTAATC	95
TBP	CGGTCGCGTCATTTTCTC	GGGTTATCTTCACACACCATGA	107