Nonylphenol and Octylphenol Differently Affect Cell Redox Balance by Modulating the Nitric Oxide Signaling

Figure 1

Cell viability of HepG2 cells exposed to octylphenol (OP) and nonylphenol (NP) measured with the MTT assay. (a) The OP dose-response on the HepG2 cell viability (24 h incubation). (b) The NP dose-response on the HepG2 cell viability (24 h incubation). (c) The time-response on the HepG2 cell viability after the incubation with OP (10 μM) and NP (20 μM). Data ± SEM; . eNOS and iNOS gene expression in response to OP and NP. (d) The gene expression of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) has been evaluated in HepG2 cells after 24 h incubation with OP (10 μM) and NP (20 μM). Relative expression was calculated versus untreated cells (CTR), after β-actin normalization. Data ± SEM; . values were considered statistically significant by ANOVA.

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