Nonylphenol and Octylphenol Differently Affect Cell Redox Balance by Modulating the Nitric Oxide Signaling

Figure 4

Oxidative and nitrosative stress induced by OP and NP. Treated and untreated HepG2 cells were assayed for (a) the intracellular ROS production of 10⁶ cells/mL by 2,7-dichlorodihydrofluorescein diacetate (DCFDA). Data are expressed as percentage of the control values, after normalization for total protein content. Data are the means ± SEM; . values were considered statistically significant by ANOVA. (b) Evaluation of nitrite-nitrate (NOx) accumulation in the cell medium after incubation with 2,3-diaminonaphthalene (DAN) (data ± SEM; ). values were considered statistically significant by ANOVA. (c) 3-Nitrotyrosine (3-NT) quantification by nitrotyrosine competitive ELISA assay on cell lysates. Data values are the means ± SEM; . values were considered statistically significant by ANOVA.

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