Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK-52E Cells Attenuates Oxalate-Induced Intracellular Oxidative Stress

Figure 2

High Slc26a6 expressed in NRK cells promotes oxalate-induced cell injury. Different cell types were treated with 700 μM oxalate. (a) Cell viability was measured using CCK-8 assay, and growth of NRK-Slc26a6 cells decreased more significantly than that of NRK-52E while NRK-siR attenuated this reduction. Data are (). . (b) LDH release was measured to evaluate the cell toxicity of oxalate, and the NRK-Slc26a6 group showed more toxicity than controls did. Data are (). . (c) Representative images of apoptotic cells at 48 h, detected using flow cytometry before and after oxalate treatment. PI: propidium iodide. (d) Cell death changes are expressed as in the column graph (). .

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