Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK-52E Cells Attenuates Oxalate-Induced Intracellular Oxidative Stress

Figure 4

Higher Slc26a6 expression induced more intracellular ROS generation by oxalate. (a) Four cell types were stimulated with oxalate (700 μM) for 3 h, and intracellular ROS levels were determined by fluorescence intensity of DCFH analyzed using flow cytometry. Changes before and after oxalate treatment are shown on the right graph as (). . (b) Lipid peroxidation was assessed by detecting the MDA level in supernatants of NRK cell lysates. NRK-Slc26a6 cells showed an obvious increase in MDA generation compared with control groups after oxalate treatment (). (c) Activity change of superoxide dismutase (SOD) expressed as . In the NRK-Slc26a6 group, SOD activity was markedly decreased compared to that in control groups. (d, e, f) Expressions of NADPH oxidase 2 (Nox2) and Nox4 before and after oxalate treatment in all groups using Western blot analysis. After oxalate treatment (700 μM) for 24 h, the expression of Nox4 increased in the NRK-Slc26a6 group and that of Nox2 was decreased in the NRK-siRNA group. Data are (), .

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