Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK-52E Cells Attenuates Oxalate-Induced Intracellular Oxidative Stress

Figure 8

Proposed mechanisms of high Slc26a6-induced injury of renal tubular epithelium cells. (a) Slc26a6 expressed on the membrane of NRK cells attracts more calcium oxalate monohydrate (COM) adhesion to cells. (b) In renal tubular epithelium cells, high expression of Slc26a6 mediates more oxalate absorption by sulfate-oxalate exchange, leading to NADPH oxidase (Nox) change and intracellular ROS generation. Overproduced ROS could lead to NFκB activation, which upregulates OPN expression. OPN plays a crucial role in the adhesion process of COM to cells. Excess COM adhesion induces cell apoptosis or necrosis, producing vesicles and crystal nucleation.

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