Propofol Protects Hippocampal Neurons from Hypoxia-Reoxygenation Injury by Decreasing Calcineurin-Induced Calcium Overload and Activating YAP Signaling

Figure 5

Propofol dephosphorylated YAP through the RhoA-Lats1 signaling pathway. (a) Starved HT-22 cells were treated with propofol (15 μmol/l) for 4 hr, and pMST1/2, total MST1/2, pLats1, and Lats1 were analyzed by Western blot. Representative results are shown from three independent experiments. ^a versus control. (b) Cells were pretreated with PTX (100 ng/ml, 16 hr), C3 transferase (1 μg/ml for 2 hr), and Y27632 (10 μmol/l for 2 hr) or transfected with different dn plasmids for 48 hr, then starved and treated with propofol (15 μmol/l for 4 hr). Cell lysates were analyzed by Western blot. Representative results are shown. (c) HT-22 cells were transfected with different dn-Rho plasmids, then starved and treated with propofol (15 μmol/l for 4 hr). Expression of pLats1 and Lats1 was analyzed by Western blot.

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