Di-(2-Ethylhexyl) Phthalate Increases Obesity-Induced Damage to the Male Reproductive System in Mice

Table 1

The reproductive organs weight, sperm motility, count, and retroperitoneal, and epididymal fat weight in 12 weeks ( ± SD).

Group	n	Relative Tes. weight (g/100 g)	Relative epididymis weight (g/100 g)	Relative Sem. weight (g/100 g)	Relative liver weight (g/100 g)	Relative kidney weight (g/100 g)	Relative Epi fat (g/100 g)	Relative Ret fat (g/100 g)	Sperm motility (%)	Sperm count (×10^6/ml)
Control	10	0.85 ± 0.06	0.29 ± 0.02	0.98 ± 0.11	4.48 ± 0.30	1.36 ± 0.10	1.25 ± 0.37	0.26 ± 0.09	31.03 ± 3.08	4.75 ± 0.50
DEHP	10	0.71 ± 0.06^b	0.27 ± 0.01^b	0.96 ± 0.14	3.97 ± 0.24^b	1.24 ± 0.04^b	2.47 ± 0.61^b	0.69 ± 0.16^b	23.47 ± 2.24^b	4.05 ± 0.44^b
DIO	10	0.74 ± 0.07^b	0.26 ± 0.01^b	0.94 ± 0.11	3.83 ± 0.16^b	1.24 ± 0.07^b	3.04 ± 0.82^b	0.92 ± 0.37^b	23.33 ± 3.94^b	3.89 ± 0.41^b
DIO + DEHP low	10	0.69 ± 0.08^b	0.26 ± 0.01^b	0.95 ± 0.09	3.73 ± 0.20^bc	1.20 ± 0.07^b	2.35 ± 0.39^bf	0.68 ± 0.16^bef	20.49 ± 2.03^bce	3.60 ± 0.44^bc
DIO + DEHP middle	10	0.69 ± 0.14^b	0.25 ± 0.02^bc	0.95 ± 0.18	3.52 ± 0.20^bdf	1.17 ± 0.03^bce	1.84 ± 0.50^adf	0.45 ± 0.15^af	19.19 ± 1.17^bdf	3.51 ± 0.42^bc
DIO + DEHP high	10	0.69 ± 0.05^b	0.23 ± 0.02^bdfhj	0.89 ± 0.16	3.32 ± 0.23^bdf	1.14 ± 0.05^bdf	1.70 ± 0.25^df	0.42 ± 0.13^df	17.15 ± 1.78^bdf	3.42 ± 0.50^bde

Data are mean ± SD. denotes statistical significance compared with the control group; and denote statistical significance compared with the DEHP group; and denote statistical significance compared with the DIO group; denotes statistical significance compared with the DIO + DEHP low group; denotes statistical significance compared with the DIO + DEHP middle group. Testis: Tes; seminal vesicles: Sem; retroperitoneal: Ret. epididymal: Epi. Relative Tes. weight = testis weight/body weight × 100. Relative epididymis weight = epididymis weight/body weight × 100. Relative Sem. weight = seminal vesicle weight/body weight × 100. Sperm motility = total motile sperm/all count sperm × 100. Relative liver weight = liver weight/body weight × 100. Relative kidney weight = kidney weight/body weight × 100. Relative Ret fat weigh = retroperitoneal fat weight/body weight × 100. Relative Epi fat weight = epididymal fat weight/body weight × 100.