N-Acetylcysteine Reduces ROS-Mediated Oxidative DNA Damage and PI3K/Akt Pathway Activation Induced by Helicobacter pylori Infection

Figure 2

ROS regulates the PI3K/Akt pathway, which is activated by H. pylori infection. (a) Gastric epithelial cells (GES-1) were incubated with H. pylori (multiplicity of infection (MOI) = 100) for various lengths of time. Immunoblots of GSK-3β, p-GSK-3β (Ser9), Akt, p-Akt (Ser473), APE-1, and beta-actin expression. (b) GES-1 cultures were incubated for 6 h at various H. pylori MOI. (c–f) GES-1 cultures were pretreated with LY294002 or NAC and then were incubated for 6 h with H. pylori (MOI = 300). Representative immunoblots and quantification of the above protein levels; .

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