Isobavachalcone Induces ROS-Mediated Apoptosis via Targeting Thioredoxin Reductase 1 in Human Prostate Cancer PC-3 Cells

Figure 3

IBC activates ER stress, which contributes to the IBC-induced lethality of PC-3 cells. Cells were exposed to 15, 30, or 45 μM IBC for 24 h. (a) Quantitation of levels of GRP78, XBP-1, CHOP, and ATF4 mRNA by RT-qPCR. (b) Protein levels of GRP78, p-eIF2α, ATF4, Chop, eIF2α, pro-caspase-3, and cleaved caspase-3 were determined by Western blot. (c) Quantitation of GRP78, p-eIF2α, eIF2α, ATF4, Chop, pro-caspase-3, and cleaved caspase-3 protein levels. All data are presented as mean ± S.D. and compared to the control group.

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