Research Article
The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro
Figure 2
DAU inhibited APP processing and Aβ accumulation. Levels of Aβ1–42 (a, b), Aβ1–40 (c, d), and Aβ1–42/Aβ1–40 (e, f) of cell lysates (a, c, e) and cell culture media (b, d, f) as a function of DAU concentration were determined by ELISA. Levels of t-APP, p-APP, s-APPα, s-APPβ, BACE1, and PS1 were determined by Western blot analysis (g, h). β-Actin was used as a loading control. . Data show the mean ± SEM. and compared to N2a/WT cells. , , and compared to untreated N2a/APP cells.
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