The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro

Figure 7

DAU reduced oxidative stress and ER stress of N2a/APP cells. (a) 8-OHdG (in green) immunostaining of N2a/WT and N2a/APP cells. DAPI (in blue) stained the cell nuclei. Proteins PRDX4 (b), PDIA1 (c), GRP75 (d), GRP78 (e), p-PERK (f), p-eIF2α, eIF2α (g), ATF-4 (h), and CHOP (i) in N2a/WT and N2a/APP cells were determined by Western blot analyses. β-Actin was used as a loading control for PRDX4, GRP78, GRP75, and PDIA1. α-Tubulin was used as a loading control for ATF-4, CHOP, and p-PERK. Total eIF2α was used as a loading control for p-eIF2α. . Data show the mean ± SEM. , , , and compared to N2a/WT cells. , , and compared to untreated N2a/APP.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)