Research Article
The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro
Table 1
The primary antibodies used in this study.
| | Antibody | Cat. | RRID | Type | Dilution | Source |
| | t-APP | ab32136 | AB_2289606 | Rabbit | 1 : 3000 | Abcam | | p-APP | #6986 | BDSC_6986 | Rabbit | 1 : 1000 | Cell Signaling | | sAPPα | 11088 | Unknown | Mouse | 1 : 50 | Immuno-Biological | | sAPPβ | 10321 | Unknown | Mouse | 1 : 50 | Immuno-Biological | | BACE1 | #5606 | AB_1903900 | Rabbit | 1 : 1000 | Cell Signaling | | PS1 | #5643 | AB_10706356 | Rabbit | 1 : 1000 | Cell Signaling | | pS396 | ab109390 | AB_10860822 | Rabbit | 1 : 20000 | Abcam | | pS404 | sc-12952 | RRID:AB_656753 | Goat | 1 : 3000 | Santa Cruz | | pT231 | 355200 | AB_2533210 | Mouse | 1 : 1000 | Thermo Fisher | | pS262 | 44750G | AB_2533743 | Rabbit | 1 : 1000 | Thermo Fisher | | Tau 1 | MAB3420 | AB_94855 | Mouse | 1 : 200000 | Millipore | | Tau 5 | ab80579 | AB_1603723 | Mouse | 1 : 3000 | Abcam | | p-GSK3α/β | #9331 | AB_329830 | Rabbit | 1 : 1000 | Cell Signaling | | GSK3α/β | #5676 | AB_10547140 | Rabbit | 1 : 1000 | Cell Signaling | | p-PP2A (Y307) | AF3989 | AB_2169636 | Rabbit | 1 : 1000 | R&D Systems | | PP2A | #2259 | AB_10695752 | Rabbit | 1 : 1000 | Cell Signaling | | p35/25 | #2680 | AB_1078214 | Rabbit | 1 : 1000 | Cell Signaling | | CDK5 | ab40773 | AB_726779 | Rabbit | 1 : 3000 | Abcam | | GRP78 | sc-376768 | Unknown | Mouse | 1 : 1000 | Santa Cruz | | GRP75 | sc-133137 | AB_2120468 | Mouse | 1 : 1000 | Santa Cruz | | PDIA1 | ab2792 | AB_303304 | Mouse | 1 : 1000 | Abcam | | HMGB1 | ab79823 | AB_1603373 | Rabbit | 1 : 50000 | Abcam | | 14-3-3-z | ab155037 | Unknown | Rabbit | 1 : 3000 | Abcam | | PRDX4 | ab16943 | AB_443567 | Rabbit | 1 : 1000 | Abcam | | 8-OHdG | ab10802 | AB_297482 | Goat | 1 : 400 | Abcam | | p-PERK | #3179 | AB_2095853 | Rabbit | 1 : 1000 | Cell Signaling | | p-eIF2α | #3597 | RRID:AB_390740 | Rabbit | 1 : 1000 | Cell Signaling | | eIF2α | #5324 | AB_10692650 | Rabbit | 1 : 1000 | Cell Signaling | | ATF-4 | #11815 | AB_2616025 | Mouse | 1 : 1000 | Cell Signaling | | CHOP | #2895 | AB_2089254 | Rabbit | 1 : 1000 | Cell Signaling | | β-Actin | sc-47778 | AB_626632 | Mouse | 1 : 3000 | Santa Cruz | | α-Tubulin | sc-73242 | AB_1130901 | Mouse | 1 : 3000 | Santa Cruz |
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