Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2

<div>Simvastatin upregulates <i>HMOX1</i> mRNA and increases HO-1 protein in HAoSMC. (a) Simvastatin influence on HAoSMC viability after 24–48 h of stimulation (MTT assay). (b) mRNA level of <i>HMOX1</i>, <i>GCLM</i>, and <i>NQO1</i> in HAoSMC after simvastatin treatment (1 <i>μ</i>M and 10 <i>μ</i>M) for 6 h and 24 h. (c) Immunofluorescent staining of HO-1 (green) and Bach1 (red) in HAoSMC after stimulation with simvastatin (1 and 10 <i>μ</i>M) for 6 h. (d) Protein expression of HO-1 and Nrf2 after 24 h stimulation with simvastatin (1 and 10 <i>μ</i>M) presented in representative Western blot. 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Oxidative Medicine and Cellular Longevity

fig3

Figure 3

Figure 3: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2 