Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2

<div>Simvastatin does not influence <i>HMOX1</i> via Nrf2/ARE system in HAoSMC. (a) Nrf2 localization after 1 h stimulation with simvastatin (1 and 10 <i>μ</i>M) or sulforaphane (10 <i>μ</i>M, positive control). Confocal microscopy: Nrf2 (red), nuclei (blue), and tubulin (green) (Mag. 400x). (b) Changes in gene expression of <i>NQO1</i> and <i>HMOX1</i> in HAoSMC with transcriptionally inactive Nrf2 (Ad-Nrf2-DN) stimulated for 6 h with simvastatin (1 and 10 <i>μ</i>M). (c) Efficiency of Nrf2 silencing in HAoSMC transfection with siNFE2L2 (Nrf2) and changes in expression of <i>NQO1</i> and <i>HMOX1</i> after 6 h stimulation with simvastatin (1 and 10 <i>μ</i>M). Data are presented as mean ± SE; <span class="nowrap"><svg height="13.532pt" id="M49" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 50.6052 13.532" width="50.6052pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z" id="g50-43"></path></g><g transform="matrix(.013,0,0,-0.013,6.079,0)"><path d="M570 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499V114C205 42 199 34 109 27V0H384Z" id="g113-50"></path></g></svg> versus siNFE2L2/AdGFP; <svg height="14.9569pt" id="M52" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -11.5275 49.4726 14.9569" width="49.4726pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z" id="g58-33"></path></g><g transform="matrix(.013,0,0,-0.013,4.946,0)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 17 -231L13 -257L254 -247L259 -218L233 -216C183 -212 177 -202 189 -142L218 -1C238 -10 266 -12 283 -12C351 3 429 48 483 105C543 168 570 242 570 304ZM482 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2 