Anti-Inflammatory and Antioxidant Actions of Methyl Jasmonate Are Associated with Metabolic Modifications in the Liver of Arthritic Rats

Table 4

Effects of MeJA treatment on glycolysis and oxygen uptake in livers from control and arthritic rats. Livers from 12 h fasted rats were perfused with Krebs/Henseleit bicarbonate as described in Section 2.10. Glucose (20 mM) was infused as glycolytic substrate. The rates of lactate and pyruvate production were computed at a 38-minute perfusion time in Figure 5 (steady-state). Glycolysis was calculated as (lactate + pyruvate)/2 and expressed as μmol glucosyl units·min⁻¹·g⁻¹. ΔOxygen consumption is the increment in the oxygen consumption due to glucose infusion at a 38-minute perfusion time. C: controls treated with saline; C corn oil: controls treated with corn oil; C300: controls treated with 300 mg·kg⁻¹ MeJA; A: arthritic rats treated with saline; A corn oil: arthritic rats treated with corn oil; A300: arthritic rats treated with 300 mg·kg⁻¹ MeJA.

Groups	Parameter (μmol·min^−1·g^−1)
	Lactate production	Pyruvate production	Glycolysis	ΔOxygen consumption
C	0.48 ± 0.02^a	0.12 ± 0.01^a	0.30 ± 0.01^a	0.16 ± 0.03^a
C corn oil	0.36 ± 0.03^a	0.13 ± 0.02^a	0.26 ± 0.02^a	0.10 ± 0.05^a
C300	0.22 ± 0.03^b	0.04 ± 0.01^b	0.13 ± 0.01^b	0.13 ± 0.02^a
A	0.94 ± 0.03^c	0.24 ± 0.03^c	0.59 ± 0.03^c	0.12 ± 0.03^a
A corn oil	0.84 ± 0.07^c	0.18 ± 0.02^a,c	0.50 ± 0.03^c	0.29 ± 0.07^a
A300	0.50 ± 0.06^a	0.19 ± 0.02^a,c	0.35 ± 0.04^a	0.27 ± 0.09^a

The data are the error of the mean of 4 animals. Values with different superscript letters in the same line are different.