Anti-Inflammatory Activity of Marine Ovothiol A in an In Vitro Model of Endothelial Dysfunction Induced by Hyperglycemia

Figure 4

Effect of ovothiol A on TNF-α-induced monocyte interaction in endothelial cells. Monocyte-HUVEC adhesion in C- and GD-HUVECs untreated (basal) and incubated for 24 h with ovothiol A (10 and 50 μM) and then stimulated for 16 h with or without TNF-α (1 ng/mL). In the histogram (upper side), quantitative data express the number of U937 cells adhering within a high-power field (3.5 mm²). Each measurement is expressed as (), each consisting of 8 counts per condition. Anti-VCAM-1 and anti-ICAM-1 antibody incubation, for 1 h before the assay, has been used as the negative control. In the lower side are representative photos of C- and GD-HUVECs for each experimental condition. ANOVA and Bonferroni multiple comparison test: in C- and GD-HUVECs. Student’s t-test: basal GD- versus C-HUVECs and TNF-α GD- versus C-HUVECs.