Anti-Inflammatory Activity of Marine Ovothiol A in an In Vitro Model of Endothelial Dysfunction Induced by Hyperglycemia

Figure 7

Effect of ovothiol A on peroxynitrite and superoxide anion levels in C- and GD-HUVECs. Peroxynitrite production (a) and superoxide anion levels (b) in C- and GD-HUVECs incubated for 24 h with ovothiol A (50 μM) with or without TNF-α-stimulation (1 and 10 ng/mL) for 16 h. PMA (200 ng/mL) + Iono (50 nM) and H₂O₂ (300 μM) for 30 min before the assay are used as positive controls for endogenous peroxynitrite and superoxide anion production, respectively. Data are expressed as the MFI ratio (compared to basal C-HUVECs) of 4 independent experiments (). ANOVA and Bonferroni multiple comparison test: in C- and GD-HUVECs. Student’s t-test: in C- and GD-HUVECs, basal and TNF-α GD- versus C-HUVECs, and PMA + Iono GD- versus C-HUVECs.

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