Anacardium microcarpum Promotes Neuroprotection Dependently of AKT and ERK Phosphorylation but Does Not Prevent Mitochondrial Damage by 6-OHDA

Figure 4

Modulation of ERK, AKT and PTEN phosphorylation, PARP cleavage in response to treatment with methanolic fraction of A. microcarpum and 6-OHDA in cortical slices. Proteins were separated by SDS–PAGE and transferred to nitrocellulose membrane. Total content and phosphorylation of proteins were detected by specific antibodies, and the reaction was developed by ECL. (a) Western blotting of phosphorylated and total forms of ERK2 and AKT phosphorylation and total forms and phospho-PTEN. (b) Quantitative analysis of ERK2 phosphorylation expressed as a ratio with its respective total form. (c) Quantitative analysis of AKT phosphorylation expressed as a ratio with its respective total form. (d) Quantitative analysis of PTEN phosphorylation expressed as a ratio with β-actin. The data are expressed as fold increase related to control group and represent mean ± SE of 4 independent experiments. Statistical analysis was performed by ANOVA, followed by the Newman-Keuls test. , , and different from control group. #, ##, and ### when compared to 6-OHDA group.

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