Ginkgo biloba Leaf Extract Protects against Myocardial Injury via Attenuation of Endoplasmic Reticulum Stress in Streptozotocin-Induced Diabetic ApoE^−/− Mice

Figure 5

Effects of GBE on the cardiac histomorphological changes in diabetic ApoE^−/− mice. (a) H&E staining of cross-sectional tissue slices of the myocardium. (b) Masson’s staining of collagen in the myocardium. The blue area against the red represents collagen deposition. Arrows indicate interstitial fibers. (c, d) Immunostaining of cleaved caspase-3. The brown-yellow area represents the positive expression of cleaved caspase-3. Images of the samples incubated only with the secondary antibody were provided correspondingly. (1A, 1B) Control group; (2A, 2B) untreated diabetic group; (3A, 3B) atorvastatin group; (4A, 4B) 200 mg/kg/day GBE group; and (5A, 5B) 400 mg/kg/day GBE group. (A) 100x magnification; (B) 200x magnification. and versus the untreated diabetic group.

(a)

(b)

(c)

(d)