Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells

Figure 8

Effects of protein precipitation on the remaining levels of Res, oxy-Res, and ac-Res in the medium. (a) To evaluate the optimal time for the determination of the content of Res–FBS complex, we mixed 20 μM Res with 10 mL of DMEM containing 10% FBS and 1 mM pyruvate. After incubation was performed at different time points, 100 μL of the medium was treated with methanol to precipitate protein as in the procedures described in Materials and Methods. The remaining Res levels (%) were analyzed through HPLC. (b) To determine the effect of pyruvate on the content of Res–FBS complex, we mixed 20 μM Res with 1 mL of DMEM with or without 10% FBS and 1 mM pyruvate. After deproteinization occurred, the remaining Res levels (%) were evaluated through HPLC. (c) We mixed 200 μM Res with 1 mL of DMEM containing 1 mM pyruvate with or without 10% FBS. After deproteinization was completed, the remaining Res levels (%) were examined through HPLC. Values (mean ± standard deviation, ) without a common letter are significantly different ().

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