Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells

Table 3

Additional SOD required to inhibit Res degradation in DMEM containing FBS and catalase or pyruvate.

	SOD activity (U/mL)
	0	0.5	5	50
FBS + catalase
Day 0	100.0 ± 1.5	100 ± 2.3	100 ± 5.1	100 ± 2.6
Day 1	102.4 ± 1.6	98.4 ± 1.9	102.4 ± 2.9	97.6 ± 5.2
Day 7	101.0 ± 1.1	99.3 ± 2.7	101.3 ± 4.7	98.3 ± 2.1
FBS + pyruvate
Day 0	100 ± 1.0	100 ± 1.8	100 ± 3.6	100 ± 2.9
Day 1	97.3 ± 5.6	101.4 ± 3.7	98.6 ± 2.8	101.2 ± 2.5
Day 7	104 ± 1.9	96.5 ± 4.3	99.7 ± 3.4	97.3 ± 4.6

Res at 200 μM was incubated for 0–7 days in DMEM, which contained 3.7 g/L sodium bicarbonate, 10% FBS, and 100 U/mL catalase (FBS + catalase) or 1 mM pyruvate (FBS + pyruvate) combined with different doses of SOD at 37°C in a CO₂ incubator supplied with 5% CO₂. The remaining Res levels (%) related to the peak area of Res on day 0 were determined by HPLC. Values (mean ± standard deviation; ) in the same column without a common letter are significantly different ().