Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells

Table 5

Degradation of Res and oxy-Res in DMEM and MEM with FBS and pyruvate under regular culture conditions.

	Res (%)	Oxy-Res (%)
DMEM
Day 0	100 ± 1.8	100 ± 2.7
Day 1	104.1 ± 2.3	97.5 ± 3.0
Day 7	111.5 ± 0.5	97.3 ± 2.8
MEM
Day 0	100 ± 5.7	100 ± 4.2
Day 1	102.9 ± 2.3	98.3 ± 5.1
Day 7	102.8 ± 3.1	94.5 ± 3.7

Res and oxy-Res were incubated for 0–7 days in media, which contained 3.7 g/L sodium bicarbonate and antibiotics for DMEM, 2.2 g/L sodium bicarbonate, 0.1 mM nonessential amino acids, and antibiotics for MEM with 10% FBS and pyruvate (1 mM) at 37°C in a CO₂ incubator supplied with 5% CO₂. The remaining levels of Res and oxy-Res (%, related to the peak area on day 0) were determined by HPLC. Values (mean ± standard deviation; ) in the same column without a common letter are significantly different ().