Resveratrol Can Be Stable in a Medium Containing Fetal Bovine Serum with Pyruvate but Shortens the Lifespan of Human Fibroblastic Hs68 Cells

Table 6

Cellular uptake of Res, oxy-Res, and ac-Res for Hs68 and IMR-90 cells in the medium.

	Res (%)	Oxy-Res (%)	Ac-Res (%)
Hs68 cells
Day 0	100.0 ± 4.7	100.0 ± 3.6	100.0 ± 5.2
Day 1	96.9 ± 4.9	99.4 ± 3.3	87.6 ± 0.4
Day 3	96.2 ± 5.6	89.3 ± 1.1	86.4 ± 2.4
Day 7	93.2 ± 1.4	75.3 ± 5.0	82.7 ± 2.3
IMR-90 cells
Day 0	100 ± 5.1	100 ± 6.8	100 ± 3.0
Day 1	98.6 ± 4.0	101.2 ± 1.0	90.6 ± 1.9
Day 3	98.3 ± 3.9	93.5 ± 3.2	90.2 ± 2.1
Day 7	90.8 ± 0.9	83.5 ± 3.9	84.4 ± 1.1

Hs68 and IMR-90 cells (1 × 10⁵) were incubated with Res, oxy-Res, and ac-Res for 0–7 days in DMEM and MEM, respectively, with 10% FBS and 1 mM pyruvate under the regular culture conditions. The remaining levels of Res, oxy-Res, and ac-Res (%; related to the concentration on day 0) in the media were determined through HPLC. The standard curves of Res, oxy-Res, and ac-Res were established for quantifications. The remaining level is inversely proportional to the cellular uptake. Values (mean ± standard deviation; ) in the same column without a common letter are significantly different (). ac-Res was completely hydrolyzed to Res after day 1. As such, the uptake of ac-Res on days 1–7 was revealed by quantifying the newly formed Res peak in the calibration curve of Res.