Research Article
Oxidative Stress Induces HSP90 Upregulation on the Surface of Primary Human Endothelial Cells: Role of the Antioxidant 7,8-Dihydroxy-4-methylcoumarin in Preventing HSP90 Exposure to the Immune System
Figure 1
Cytofluorimetric analysis of HSP90 surface expression (percentages of positive cells) in HUVEC treated with H2O2 or left untreated. (a) Results expressed as mean percentages and standard error of the mean (). ; ; ; ; ; . (b) Representative histograms showing HSP90 surface expression in HUVEC treated with 300, 600, and 800 μM of H2O2 or left untreated. M1: negative cells; M2: positive cells. Samples labelled with only antirabbit IgG-Alexa Fluor 488 antibody were used as negative controls of the cytofluorimetric assay.
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