Mild Hyperthermia-Induced Myogenic Differentiation in Skeletal Muscle Cells: Implications for Local Hyperthermic Therapy for Skeletal Muscle Injury
Mild heat treatment promotes myoblast proliferation but not migration. (a and b) Migrating myoblasts were observed under phase contrast microscope (magnification = 20x). Scale bar represents 50 μm. After scratch onto 60 mm culture plates with a 200 μm pipette tip at 95% confluency, migrating myoblasts were measured 24 hours after scratch and were expressed as % of NT. (c) The trypsin-treated myoblasts suspended in medium were counted with hemocytometer 48 hours after incubation in DMEM supplemented with 10% FBS under specified temperature condition. The data were expressed as cells/mL. Each experiment was performed with at least four separate cell cultures. The bar graph represented means ± SEM. The data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. versus NT (no treatment); versus IHT (intermittent heat treatment).