Mild Hyperthermia-Induced Myogenic Differentiation in Skeletal Muscle Cells: Implications for Local Hyperthermic Therapy for Skeletal Muscle Injury
Mild heat treatment promotes the expressions of MyHCI and myogenin in a treatment time-dependent manner. (a) The gene expressions of myosin heavy chain isoforms (MyHCI, IIa, IIx, and IIb) and (b) myogenic factor (myogenin) were quantified in myotubes differentiated under specified temperature condition. Each experiment was performed with at least four separate cell cultures. Bar graph represented means ± SEM. The data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. versus NT (no treatment); versus IHT (intermittent heat treatment).