Mild Hyperthermia-Induced Myogenic Differentiation in Skeletal Muscle Cells: Implications for Local Hyperthermic Therapy for Skeletal Muscle Injury
Mild heat treatment promotes myogenic differentiation in a PGC-1α dependent manner. (a) Myoblasts were transfected with control siRNA or Pgc-1α siRNA and were incubated to differentiate for 72 hours under specified temperature condition in DMEM supplemented with 2% horse serum, 100 units/mL penicillin, and 100 mg/mL streptomycin. Differentiated myotubes were stained with DAPI and were visualized by fluorescence microscope (magnification = 20x). Scale bar represents 50 μm. (b) Myotube width, (c) myotube length, and (d) the levels of myogenic expression were quantified. Each experiment was performed with at least four separate cell cultures. Bar graph represented means ± SEM. The data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. versus untreated cells.