Indoleamine 2,3-Dioxygenase-Dependent Neurotoxic Kynurenine Metabolism Contributes to Poststroke Depression Induced in Mice by Ischemic Stroke along with Spatial Restraint Stress

Figure 4

Double immunostaining for IDO1 and NeuN, GFAP, or Iba-1 in PSD mice. (a) Immunofluorescence of IDO1 (red) and NeuN (green), a neuronal marker of the nucleus accumbens, hippocampus, and hypothalamus in PSD mice. The yellow dashed rectangle marks the position of an enlarged image showing double immunostaining of IDO1 and NeuN. (b) Immunofluorescence of IDO1 (red) and GFAP (green), an astrocyte marker in PSD mice. The yellow dashed rectangle marks the position of an enlarged image showing double immunostaining of IDO1 and GFAP. (c) Immunofluorescence of IDO1 (red) and Iba-1 (green), a microglial marker in PSD mice. The yellow dashed rectangle marks the position of an enlarged image showing double immunostaining of IDO1 and Iba-1. Arrows indicate the colocalization of IDO-1(+)/Iba-1(+) immunofluorescence (yellow). Nuclear DNA was labeled with blue fluorescence by DAPI. Scale .

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