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Oxidative Medicine and Cellular Longevity
Volume 2018 (2018), Article ID 2479602, 14 pages
https://doi.org/10.1155/2018/2479602
Research Article

Pretreatment with Shuanghe-Tang Extract Attenuates Postischemic Brain Injury and Edema in a Mouse Model of Stroke: An Analysis of Medicinal Herbs Listed in Dongui Bogam

1Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea
2Korean Medical Science Research Center for Healthy Aging, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea
3Graduate Training Program of Korean Medicine for Healthy Aging, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea
4Division of Humanities and Social Medicine, School of Korean Medicine, Pusan National University, Yangsan, Gyeongnam 50612, Republic of Korea

Correspondence should be addressed to Jin Ung Baek; rk.ca.nasup@nagajkum and Hwa Kyoung Shin; rk.ca.nasup@eiluj

Received 1 August 2017; Accepted 31 October 2017; Published 11 February 2018

Academic Editor: Angel Catalá

Copyright © 2018 Min Jae Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. Although stroke is among the leading causes of death and long-term disability, there are few effective treatments for limiting the severity of neurological sequelae. We evaluated the effects of 29 medicinal herbs listed in the Pung chapter of the 17th century Korean medical text Dongui Bogam on stroke symptoms in a mouse model of cerebral ischemia. Methods. Focal cerebral ischemia was induced via photothrombosis. Infarct volume, brain edema, and neurological deficits were evaluated. Immunofluorescence staining for tight junction proteins and aquaporin 4 (AQP4) was performed following ischemic injury. Results. Based on our initial findings, we examined the effects of two prescriptions in which the candidate herbs comprised more than 60% of the total formula: Shuanghe-tang and Zengsunsiwu-tang. Pretreatment with Shuanghe-tang significantly reduced infarct volume, decreased blood-brain barrier (BBB) breakdown, attenuated edema, and improved neurological and motor functions in a dose-dependent manner (30, 100, and 300 mg/kg), while no such effects were observed in mice pretreated with Zengsunsiwu-tang. Immunohistochemical analysis revealed significant increases in ipsilateral occludin and zonula occludens 1 (ZO-1) expression in Shuanghe-tang-pretreated mice, as well as increased AQP4 immunofluorescence. Conclusions. These results indicate that Shuanghe-tang may protect against brain injury and promote recovery of neurological function following ischemia.