The Effect of Chronic NO Synthase Inhibition on the Vasoactive and Structural Properties of Thoracic Aorta, NO Synthase Activity, and Oxidative Stress Biomarkers in Young SHR

Figure 6

ROS production and structural changes (elastin arrangement and wall thickness) in the TA after L-NAME treatment in young SHR. DHE fluorescence detection (ex 540 nm/em 590 nm) was used to assess vascular redox homeostasis disruption in aortic cryosections, where ROS formation was determined by calculating the intensity (density/area) (a, b). Elastin autofluorescence (ex 488 nm/em 510 nm) was detected using fluorescence microscopy of aortic cryosections (c, d). Wall thickness: tunica intima + tunica media was determined in μm (e, f). E: endothelium; M: tunica media; A: adventitia; SHR: control group (); SHR + L-NAME: SHR treated with L-NAME ().

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