Formyl Peptide Receptor 1 Modulates Endothelial Cell Functions by NADPH Oxidase-Dependent VEGFR2 Transactivation

Figure 6

Wound healing and capillary-like network formation induced by FPR1. (a) Representative images (left) and bar graph quantification (right) of ECV304 cell migration from 5 independent experiments. Cells were incubated with 0.1 μM N-fMLP or vehicle in the presence or absence of PTX. Images were acquired at different times (0, 24, and 36 hours) after wound injury (scale bar: 20 μm). (b) Capillary-like network formation (left) was performed in Matrigel-coated plates. ECV304 cells were incubated with N-fMLP in the presence or absence of PTX, and bar graphs (right) show the quantification of relative tube length from four independent experiments. Cells incubated with VEGF (20 μg/ml) were used as a positive control (scale bar: 50 μm). compared with unstimulated cells.

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