Superoxide Anion Production and Bioenergetic Profile in Young and Elderly Human Primary Myoblasts

Figure 2

SOD1 and SOD2 protein expression. Western blotting analysis of superoxide enzymes cytosolic type 1 and mitochondrial type 2 was performed on young and elderly myoblasts and myotubes. β-Actin content was used for normalization. Panels (a), (b), (c), and (d) show SOD1 and SOD2 densitometric analysis of Western blots performed on three young and three old samples expressed as . Representative patterns of SOD1, SOD2, and β-actin (as loading control) expression in young and elderly myoblasts and myotubes are shown in panels (e) and (f). The bands were taken from two nonadjacent lanes originating from exactly the same gel and blot with exactly the same exposure time, but spliced together indicated with double-dotted lines. Moreover, we performed no change in contrast ().

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