Polydatin Protects Diabetic Heart against Ischemia-Reperfusion Injury via Notch1/Hes1-Mediated Activation of Pten/Akt Signaling
Effect of DAPT and LY294002 on myocardial injury in sham-operated diabetic rats. The diabetic animals received sham operation in the presence or absence of DAPT or LY294002 treatment. (a) Body weight of control rats and diabetic rats. The data was collected before and during the induction of diabetes (7 days). Before the MI/R surgery, the body weight was measured once again. (b) Bar diagram showing nonfasting plasma glucose of control rats and diabetic rats after 7 days of streptozotocin injection. (c) Bar diagram showing fasting plasma glucose of control rats and diabetic rats after 7 days of streptozotocin injection. (d) Intraperitoneal glucose tolerance test after 7 days of streptozotocin injection. (e) Oral glucose tolerance test (OGTT) after 7 days of streptozotocin injection. (f) Bar diagram showing left ventricular systolic pressure. (g) and (h) Bar diagram showing first derivative of left ventricular pressure (+dP/dtmax and −dP/dtmax). (i) Representative photomicrographs of in situ detection of apoptotic myocytes by TUNEL staining (×200, bar = 200 μm). Apoptotic nuclei were stained with TUNEL (row 1, green staining). Total nuclei were stained with DAPI (row 2, blue staining). Cardiomyocytes were stained with anti-α-sarcomeric actin (row 3, red staining). (j) Bar diagram showing apoptotic index (percentage of TUNEL-positive nuclei). (k) Bar diagram showing cleaved caspase-3 expression. Top images: representative blots. (l) Bar diagram showing myocardial infarct size. Top images: representative photographs of heart sections. Blue-stained area indicates nonischemic, normal region; red-stained area, ischemia-reperfused but not infarcted region; and negative-stained area, ischemia-reperfused infarcted region. animals or samples per group. / versus the control group. DM, diabetes mellitus; PG, plasma glucose; IPGTT, intraperitoneal glucose tolerance test; OGTT, oral glucose tolerance test; MI/R, myocardial ischemia-reperfusion; LY, LY294002; LVSP, left ventricular systolic pressure; AAR, area at risk; INF, infarct area.
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