Polydatin Protects Diabetic Heart against Ischemia-Reperfusion Injury via Notch1/Hes1-Mediated Activation of Pten/Akt Signaling
Effect of LY294002 on cardiac function and myocardial damage in MI/R-injured diabetic rats. TUNEL staining and Evans blue-TTC double staining were performed after 6 hours of reperfusion. The rest of the measurements was carried out after 3 hours of reperfusion. (a) Bar diagram showing left ventricular systolic pressure. (b) and (c) Bar diagram showing first derivative of left ventricular pressure (+dP/dtmax and −dP/dtmax). (d) Representative photomicrographs of in situ detection of apoptotic myocytes by TUNEL staining (×200, bar = 200 μm). Apoptotic nuclei were stained with TUNEL (row 1, green staining). Total nuclei were stained with DAPI (row 2, blue staining). Cardiomyocytes were stained with anti-α-sarcomeric actin (row 3, red staining). (e) Bar diagram showing apoptotic index (percentage of TUNEL-positive nuclei). (f) Bar diagram showing cleaved caspase-3 expression. Top images: representative blots. (g) Bar diagram showing caspase-3 activity. (h) Bar diagram showing myocardial infarct size. Top images: representative photographs of heart sections. Blue-stained area indicates nonischemic, normal region; red-stained area, ischemia-reperfused but not infarcted region; and negative-stained area, ischemia-reperfused infarcted region. (i) and (j) Bar diagram showing plasma CK and LDH activity. The results are expressed as the mean ± SEM. animals or samples per group. / versus the sham group and / versus the MI/R + PD group. MI/R, myocardial ischemia-reperfusion; PD, polydatin; LY, 294002; LVSP, left ventricular systolic pressure; AAR, area at risk; INF, infarct area; CK, creatine kinase; LDH, lactate dehydrogenase.
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