Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 5

Research Article

Mangiferin and Morin Attenuate Oxidative Stress, Mitochondrial Dysfunction, and Neurocytotoxicity, Induced by Amyloid Beta Oligomers

Figure 5

Effects of morin and mangiferin on antioxidant enzyme activities and protein oxidation in Aβ-treated neurons. (a) Neurons were pretreated with morin and mangiferin (1 μM, 30 min) and then exposed to 5 μM Aβ for 2 h. Cells were then harvested and lysed for the determination of levels of catalase and SOD. Data () from 4 experiments were normalized to the enzyme activity of vehicle-treated cells. compared with vehicle-treated cells, , , compared with Aβ-treated cells. (b, c) Detection of protein carbonyls on neuronal protein extracts was performed by immunoblotting assays based on the use of antidinitrophenyl (DNP) antibody. The Western blot analysis of dinitrophenyl content of 12 μg of proteins illustrates the effects of Aβ (5 μg, 2 h) together with antioxidants in protein carbonyl levels. Graph bars represent the intensities of bands normalized to total protein load (, cultures) displayed as a percentage of untreated cells. compared with untreated cells; compared with Aβ-treated cells.

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