Phytochemical Analysis of Anti-Inflammatory and Antioxidant Effects of Mahonia aquifolium Flower and Fruit Extracts

Figure 1

(a) Chromatograms at 280 nm of the M. aquifolium flower, green fruit, and ripe fruit extracts. The ten standards are indicated by arrows and numbers. Berbamine (7) is barely visible in the 280 nm chromatogram, but it is much better detected and quantified separately from the 220 nm chromatogram. (b) HPLC-DAD registered absorption molecular spectra in the UV-vis domain for the polyphenolic standards at 350 μg/mL. (c) HPLC-DAD registered absorption molecular spectra in the UV-vis domain for the alkaloid standards at 350 μg/mL. 1—Chlorogenic acid, 2—p-coumaric acid, 3—ferulic acid, 4—rutin, 5—isoquercitrin, 6—quercetin, 7—berbamine, 8—jatrorrhizine, 9—palmatine, and 10—berberine. Sample 1—Mahonia aquifolium flowers, sample 2—Mahonia aquifolium ripe fruits, and sample 3—Mahonia aquifolium green fruits.

(a)

(b)

(c)