NLRP3 Inflammasome Formation and Activation in Nonalcoholic Steatohepatitis: Therapeutic Target for Antimetabolic Syndrome Remedy FTZ

Figure 5

Nlrp3 gene silencing inhibited PA-induced NLRP3 inflammasomes formation and activation in HSCs with and without FTZ treatment. (a) Representative fluorescence confocal microscopic images showing the colocalization of ASC with caspase-1. (b) Correction coefficient (PCC) showing a statistically significant increase in colocalization of ASC with caspase-1 in PA-treated HSCs, which was suppressed by Nlrp3 siRNA and FTZ. (c) Representative Western blot gel documents showing cleaved caspase-1 increased in HSCs treated with PA but blocked by Nlrp3 siRNA and FTZ. (d) Densitometric quantitation of immunoreactive bands showing a statistically significant decrease in cleaved caspase-1 by using Nlrp3 siRNA and FTZ ( batches of cells). versus Ctrl-scram group; versus PA-scram group.

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