Research Article
NLRP3 Inflammasome Formation and Activation in Nonalcoholic Steatohepatitis: Therapeutic Target for Antimetabolic Syndrome Remedy FTZ
Figure 7
Superoxide production and NADPH oxidase-membrane raft (MR) clustering in HSCs induced by PA with and without FTZ. (a) Representative ESR traces of superoxide (O2•−) trapped by CMH using NADPH as substrate upon PA stimulation in HSCs. (b) The bar graph summarizing ESR data, showing that PA enhanced O2•− production in HSCs, which was attenuated by FTZ and NAC ( batches of cells). (c) Representative fluorescence confocal microscopic images showing the colocalization of MR component labeled by CTXB with NADPH oxidase subunit gp91 or p47. (d) Correction coefficient (PCC) showing a statistically significant increase in colocalization of CTXB with gp91 or p47 in PA-treated HSCs, which was suppressed by FTZ or NAC treatment ( batches of cells). versus Ctrl-Vehl group; versus PA-Vehl group.
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