Characterization of the Antioxidant Effects of <i>γ</i>-Oryzanol: Involvement of the Nrf2 Pathway

<div>Oryzanol protects from cell death and decreases H<sub>2</sub>O<sub>2</sub>-induced ROS/RNS generation. (a) HEK-293 cells were pretreated for 24 h with ORY (ORY<sub>24h</sub>) at different concentrations and then stressed by the addition of 100 <i>μ</i>M H<sub>2</sub>O<sub>2</sub> for 24 h. Cell viability was evaluated with MTT assay. Data are shown as percentage of cell viability compared with untreated cells; <svg height="13.532pt" id="M12" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 68.0304 13.532" width="68.0304pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z" id="g50-43"></path></g><g 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id="g113-54"></path></g></svg> versus H<sub>2</sub>O<sub>2</sub> group. (b) Effects of 5 <i>μ</i>g/mL ORY<sub>24h</sub> on H<sub>2</sub>O<sub>2</sub>-induced ROS/RNS production were determined by H<sub>2</sub>DCF-DA oxidation using a fluorescence microplate reader. Fluorescence intensity of ORY<sub>24h</sub> (open square symbol) after oxidative insult significantly decreased over time with <svg height="13.532pt" id="M15" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 56.1861 13.532" width="56.1861pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z" 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Bonferroni’s multiple comparison test was used.</div>

Oxidative Medicine and Cellular Longevity

fig1

Figure 1

Figure 1: Characterization of the Antioxidant Effects of <i>γ</i>-Oryzanol: Involvement of the Nrf2 Pathway 

Oxidative Medicine and Cellular Longevity

Characterization of the Antioxidant Effects of γ-Oryzanol: Involvement of the Nrf2 Pathway

Figure 1